Table 3. Lymphocyte sub-set phenotypes in CF subjects based on the presence of P. aeruginosa infection compared to healthy control subjects.
| CF, No P. aeruginosa (n = 5) | CF, P. aeruginosa infection (n = 36) | P-Value∧ | Non-CF (n = 22) | P-Value$ | |
| Sex (Female:Male) | 3∶2 | 14∶22 | 0.4 | 11∶11 | 1.0 |
| Age (years) | 23.3 (18.4–41.7) | 28 (24.5–32.6) | 0.8 | 26 (25–32) | 0.5 |
| BMI (kg/metre2) | 24.7 (19.9–31.7) | 23.4 (20.5–25.3) | 0.5 | 24.6 (21.4–28.3) | 0.8 |
| FEV1% Predicted (%) | 72.4 (59.1–88.8) | 63.6 (43.4–80.1) | 0.3 | 110 (101–120) | 0.001 |
| FVC % Predicted (%) | 83.9 (68.1–95.9) | 79.3 (71.1–88.7) | 0.5 | 107 (95–112) | 0.012 |
| Lymphocyte Population (size gated, CD14−) | |||||
| T-Cells (CD3+CD16−) | 68.2 (63.4–76.8) | 72.9 (68.3–81.3) | 0.4 | 74.9 (67.9–79.0) | 0.3 |
| CD4+CD8− | 64.4 (49.6–70.0) | 65.2 (56.9–70.5) | 0.6 | 65.1 (59.9–71.4) | 0.5 |
| CD8+CD4− | 26.2 (22.8–38.8) | 25.1 (20.5–33.1) | 0.6 | 27.4 (22.7–32.8) | 0.9 |
| MAIT Cells (CD161+, TCR Va7.2+) | 2.4 (1.4–3.6) | 1.0 (0.3–1.8) | 0.023 | 2.0 (1.4–3.1) | 1.0 |
| γ/δ T-cells (TCR γ/δ+) | 8.0 (6.0–16.0) | 10.5 (6.6–13.4) | 0.7 | 6.4 (4.6–9.4) | 0.2 |
| CD4+CD8+ | 0.2 (0.2–0.2) | 0.3 (0.2–1.1) | 0.014 | 0.4 (0.3–1.7) | 0.004 |
| CD4−CD8−* | 3.1 (1.6–4.1) | 2.1 (0.7–2.8) | 0.2 | 1.6 (1.1–2.3) | 0.1 |
| B-Cells (CD3−CD16−CD20+HLA-DR+) | 17.2 (14.0–20.9) | 8.5 (3.6–13.0) | 0.002 | 4.8 (3.9–8.7) | 0.003 |
| Non-Resting (CD1c−) | 74.5 (69.1–79.0) | 64.1 (55.5–73.7) | 0.1 | 71.6 (60.5–75.1) | 0.1 |
| Resting (CD1c+) | 24.9 (20.3–30.5) | 35.9 (25.3–43.9) | 0.1 | 28.1 (25.0–38.3) | 0.1 |
| NK-Cells (CD3−) | 9.6 (4.6–10.9) | 9.5 (7.8–12.5) | 0.5 | 13.1 (8.0–18.0) | 0.039 |
| CD16+CD56dim HLA-DR− | 69.2 (48.5–80.9) | 76.1 (63.8–88.1) | 0.2 | 88.0 (84.7–92.6) | 0.006 |
| CD16−CD56+HLA-DR− | 10.9 (7.1–14.5) | 8.5 (6.1–13.3) | 0.5 | 6.1 (4.5–9.1) | 0.1 |
| CD16−CD56+HLA-DR+ | 21.4 (10.8–37.4) | 14.2 (4.5–23.8) | 0.2 | 4.3 (2.7–9.2) | 0.006 |
| CD3+CD16+ | 2.0 (0.9–2.6) | 2.5 (1.0–4.2) | 0.4 | 1.8 (1.1–3.6) | 0.9 |
| Contaminants/Undefined | 1.8 (1.6–3.5) | 3.7 (3.1–5.4) | 0.017 | 2.4 (1.9–3.9) | 0.4 |
∧CF No P. aeruginosa versus CF P. aeruginosa infection. $CF No P. aeruginosa versus Non-CF. *After exclusion of MAIT and γ/δ T-cells. Values expressed as percentage of parent population, Median (interquartile range). Significance of between group differences determined by Mann-Whitney U test.