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. 2014 Oct 8;9(10):e110150. doi: 10.1371/journal.pone.0110150

Figure 3. cGAMP promotes antigen-specific Ig responses in immunized mice.

Figure 3

Mice were immunized with OVA alone or adjuvanted with CTB, c-di-AMP or cGAMP. Sera samples of these mice were analyzed for their OVA-specific (A) total IgG, (B) IgG1, (C) IgG2c, (D) IgA titers by ELISA. The titer gives the dilution factor of the assay sample with twice the readout value of the blank (absorbance of light of 405 nm wave length). (E) Mucosal IgA was analyzed in nasal lavage samples by ELISA and is given as percentage of OVA-specific IgA of total IgA titers. The error bars represent the SEM of three independent experiments with five mice per group per experiment. Significant differences between select groups are indicated with * for p<0.05 and ** for p<0.01.