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. 2014 Sep 24;21(1):83. doi: 10.1186/s12929-014-0083-1

Table 1.

List of primer pairs used for RT-PCR/ qPCR

Gene Primer sequence Annealing temp.
Nestin GCCCTGACCACTCCAGTTTA 55.0°C
GGAGTCCTGGATTTCCTTCC
Neurofilament TGGGAAATGGCTCGTCATTT 56.7°C
CTTCATGGAAGCGGCCACTT
TUJ I GGGATCCACTCCACGAAGTA 61.0°C
CGAGACCTACTGCATCGACA
MAP2 CTCAACAGTTCTATCTCTTCTTCA 60.0°C
TCTTCTTGTTTAAATCCTAACACT
TH (For RT & qPCR) GGTCGCGCTGCCTGTACT 53.9°C
TCATCACCTGGTCACCAAGTT
GAPDH GACAAGCTTCCCGTTCTCAG 57.0°C
GAGTCAACGGATTTGGTCGTCGT