Table 1. Population data and genetic diversity indices of 17 Chilean populations of Dioscorea humilis for eight nuclear microsatellite loci and overall estimates for taxonomic and geographical population groups.
Code | Locality | Latitude | Longitude | Altitude (m) | N 1 | A1 | H O 1 | H E 1 | F IS 1 |
Dioscorea humilis subsp. Humilis (Dhh01 to Dhh15) | 488 | 3.969 | 0.468 | 0.425 | −0.103*** | ||||
Northern D. humilis subsp. humilis (Dhh01 to Dhh08) | 257 | 3.800 | 0.466 | 0.410 | −0.136*** | ||||
Dhh01 | Valparaíso:Catapilco, rincónde La Mestiza | 32° 32′ 33.4″ S | 71° 17′ 37.7″ W | 90 | 30 | 4.125 | 0.471 | 0.405 | −0.165** |
Dhh02 | Metropolitana:Granizo, ParqueNacional de LaCampana, sectorGranizo | 32° 58′ 54.9″ S | 71° 07′ 58.2″ W | 450 | 28 | 4.000 | 0.424 | 0.386 | −0.100** |
Dhh03 | Metropolitana:Granizo, ParqueNacional de LaCampana, La Troyabridge. | 32° 59′ 05.3″ S | 71° 08′ 17.8″ W | 420 | 30 | 4.125 | 0.425 | 0.371 | −0.148** |
Dhh04 | Metropolitana: ParqueNacional de LaCampana, sectorCajón grande | 33° 00′ 06.1″ S | 71° 07′ 53.5″ W | 340 | 30 | 3.500 | 0.350 | 0.391 | +0.107* |
Dhh05 | Metropolitana:Carretera a Til Til,Entrance to Parcelaciónde El tranque. | 33° 08′ 40.7″ S | 70° 52′ 34.8″ W | 530 | 36 | 3.875 | 0.438 | 0.392 | +0.036** |
Dhh06 | Metropolitana: Roadfrom Til Til toLimache, detour toLa Vega through elCamarico. | 33° 03′ 28.3″ S | 71° 02′ 57.2″ W | 700 | 31 | 3.875 | 0.510 | 0.458 | +0.019** |
Dhh07 | Metropolitana: Santiagode Chile, CerroManquehue, creekstarting from Agua delPalo. | 33° 21′ 50.3″ S | 70° 34′ 56.9″ W | 820 | 36 | 3.625 | 0.523 | 0.423 | −0.241** |
Dhh08 | Metropolitana: Santiago de Chile, Cerro de Renca. | 33° 23′ 43.1″ S | 70° 42′ 38.9″ W | 540 | 36 | 4.500 | 0.560 | 0.453 | −0.135** |
Southern D. humilis subsp. humilis (Dhh09 to Dhh15) | 231 | 4.163 | 0.472 | 0.442 | −0.068*** | ||||
Dhh09 | Metropolitana: Maipo,Cerro Cantillana, side offorest track from Ragueto Pabellón. | 33° 51′ 10.8″ S | 70° 58′ 44.5″ W | 630 | 36 | 4.625 | 0.460 | 0.467 | +0.108** |
Dhh10 | Metropolitana : Maipo,Cerros de Aculeo. | 33° 50′ 04.4″ S | 70° 51′ 14.0″ W | 380 | 36 | 4.714 | 0.549 | 0.521 | −0.044ns |
Dhh11 | ÓHiggins: Road fromRancagua to Doñihue. | 34° 11′ 28.2″ S | 70° 50′ 39.4″ W | 430 | 30 | 5.000 | 0.432 | 0.459 | +0.078** |
Dhh12 | ÓHiggins: Road fromCoya to Pangal. | 34° 12′ 03.2″ S | 70° 30′ 58.4″ W | 850 | 29 | 3.250 | 0.453 | 0.357 | −0.177** |
Dhh13 | ÓHiggins: Road from SanFernando to Tinguiririca,next to La Rufina foresttrack. | 34° 40′ 27.0″ S | 70° 52′ 56.4″ W | 550 | 36 | 4.625 | 0.485 | 0.444 | −0.073** |
Dhh14 | Maule: Road fromCuricó to Sagrada Familia. | 35° 03′ 03.0″ S | 70° 31′ 03.2″ W | 170 | 36 | 4.875 | 0.447 | 0.413 | +0.046ns |
Dhh15 | Bio-Bio: BetweenYumbel and Monteáguila | 37° 05′ 00.4″ S | 72° 28′ 46.1″ W | 110 | 28 | 3.875 | 0.556 | 0.502 | −0.064* |
Dioscorea humilis subsp. polyanthes (Dhp01-Dhp02) | 70 | 4.131 | 0.574 | 0.480 | −0.195*** | ||||
Dhp01 | Maule: Constitución,just after the river Maulebridge to Putú. | 35° 20′ 05.6″ S | 72° 23′ 17.2″ W | 20 | 35 | 4.250 | 0.598 | 0.489 | −0.222** |
Dhp02 | Maule: de Constitucióna San Javier | 35° 26′ 24.1″ S | 72° 20′ 00.2″ W | 330 | 35 | 4.500 | 0.550 | 0.475 | −0.162ns |
N, sample size; A, mean number of alleles per locus; H O, H E, observed and expected heterozygosity, respectively; F IS, inbreeding coefficient. Significance: *, P<0.05, **, P<0.01, ***, P<0.001, ns, not significant.