Table 2.
Throughput and quality of RNA-seq of the reference library and the DGE libraries
| Libaries | Total | Total Nucleotides | Q20 percentage | N percentage | GC percentage |
|---|---|---|---|---|---|
| Reads | (nt) | ||||
| Reference library | 61,338,190 | 6,133,819,000 | 97.82% | 0.01% | 46.73% |
| Lc0h-1 | 30,382,747 | 1,488,754,603 | 98.51% | 0.01% | 46.11% |
| Lc0h-2 | 30,181,112 | 1,478,874,488 | 98.53% | 0.01% | 45.85% |
| Lc5h-1 | 28,942,560 | 1,418,185,440 | 98. 45% | 0.01% | 46.11% |
| Lc5h-2 | 32,276,093 | 1,581,528,557 | 98.48% | 0.01% | 46.28% |
| Lc10h-1 | 30,733,719 | 1,505,952,231 | 98.50% | 0.01% | 45.90% |
| Lc10h-2 | 31,382,314 | 1,537,733,386 | 98.51% | 0.01% | 45.83% |
One reference library was constructed by mixing RNA extracted from ROS-treated rudimentary leaves in 0 h, 5 h and 10 h of treatment. 6 DGE libraries were constructed from 0 h, 5 h and 10 h of ROS-treated rudimentary leaves. Each time point of treatment had 2 biological replicates. All libraries were sequenced using HiSeq 2000. Q20 percentage indicates the percentage of sequences with sequencing error rate lower than 1%. N percentage is the percentage of nucleotides which could not be sequenced.