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. 2014 Oct 3;14:243. doi: 10.1186/s12866-014-0243-1

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© Kakuda et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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β-Galactosidase activity assays of P _vapA -acZ virS and virR deletion mutant strains. β-Galactosidase activity (measured in Miller units) of the wild-type strain (TKR255), ΔvirS, pINT::P_aphII-virS-integrated ΔvirS (P_aphII-virS/ΔvirS), pINT::P_aphII-virS (D57A)-integrated ΔvirS [P_aphII-virS (D57A)/ΔvirS], virR _ΔHTH, pINT::P_virR-virR-integrated virR _ΔHTH (P_virR-virR/virR _ΔHTH), and pINT::P_aphII-virS-integrated virR _ΔHTH (P_aphII-virS/virR _ΔHTH) strains grown at pH 6.5 (lanes 1 and 3) or pH 8.0 (lanes 2 and 4). Error bars represent standard deviations for each data set (n = 3). Data were evaluated for statistical significance using one-way ANOVA followed by the Dunnett’s multiple comparison test, *p < 0.001.