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. 2014 Sep 25;11:45. doi: 10.1186/1743-7075-11-45

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© Prashanth et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of vitamin A supplementation on BAT morphology. Five-month-old male lean (A) and obese (B) rats were fed vitamin A at a dose of 2.6 (I), 26 (II), 52 (III) and 129 (IV) mg/kg diet for a period of 20 weeks. AI served as the control group for lean phenotype, while BI was the control group for obese phenotype. The BAT samples were fixed, and sections were stained with uranyl citrate and lead citrate for morphologic analysis. Photomicrographs were taken at 15000x magnification. Scale bar, 667 nm. The ruptured membranes observed in obese group (BI) are depicted by arrow heads. The intact mitochondria in BII and BIII are depicted by arrow. M- mitochondria, LD- lipid droplet.