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. 2014 Jul 31;6(3):1570–1578. doi: 10.3390/cancers6031570

Figure 1.

Figure 1

The effects of MCKH- and HDF-conditioned media (CM). (a) MCF-7, a human breast adenocarcinoma cell line, and CRL-8797, a normal mammary gland breast epithelial cell line, were cultured in freshly prepared MCF medium (control), MCKH-CM, and HDF-CM. Cell proliferation was assessed by using a 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) method and evaluated relative to proliferation in the control medium. Some p-values of the t-tests are represented; (b) The effects of tyrosine-kinase inhibitors on the activity of MCKH-CM to MCF-7. MCF-7 was cultured in the control medium, FGF-fortified medium, and MCKH-CM, with (SU5402, U-126) or without (−) inhibitors. Cell proliferation was assessed using an MTT method and evaluated relative to proliferation in the control medium without inhibitors.