Table 1.
Expression profiles of CAF-related genes in MCKH cells.
| Gene name | Gene | malignant tissue * | CRL8798 * | MCKH * | |||
|---|---|---|---|---|---|---|---|
| transcription factors | |||||||
| HIF1A | hypoxia inducible factor 1, α subunit | 1.28 ± 0.16 | 3.93 ± 0.16 | 9.40 ± 1.30 | |||
| NFKB2 | NF-κB2 | 1.04 ± 0.12 | 4.25 ± 0.50 | 2.07 ± 0.30 | |||
| BNIP3 | BCL2/adenovirus E1B interacting protein 3 | 0.77 ± 0.02 | 11.72 ± 0.23 | 20.85 ± 0.72 | |||
| glycolytic enzymes | |||||||
| GAPDH | glyceraldehyde-3-phosphate dehydrogenase | 1.08 ± 0.02 | 8.11 ± 0.23 | 16.78 ± 0.47 | |||
| LDHA | lactate dehydrogenase A | 1.05 ± 0.05 | 21.22 ± 3.08 | 32.17 ± 5.40 | |||
| PGAM1 | phosphoglycerate mutase 1 | 1.3 ± 0.50 | 10.84 ± 0.49 | 17.22 ± 2.79 | |||
| PGK1 | phosphoglycerate kinase 1 | 1.35 ± 0.04 | 10.83 ± 0.19 | 15.67 ± 0.62 | |||
| ALDOA | aldolase A, fructose-bisphosphate | 1.81 | 5.95 | 8.39 | |||
| ENO1 | enolase 1, α | 0.58 | 12.00 | 10.15 | |||
| PKM2 | pyruvate kinase, muscle | 1.31 | 11.59 | 18.56 | |||
| TPI1 | triosephosphate isomerase 1 | 1.06 | 9.04 | 13.87 | |||
| transporters | |||||||
| SLC16A1 | monocarboxylic acid transporter 1 | 0.97 | 14.34 | 12.03 | |||
| SLC16A3 | monocarboxylic acid transporter 4 | 5.44 | 116.02 | 136.50 | |||
| SLC1A5 | neutral amino acid transporter | 0.50 | 4.16 | 25.87 | |||
| others | |||||||
| COLA2 | collagen, type I, α2 | 26.20 ± 1.70 | 0.0006 ± 0.0002 | 35.32 ± 2.13 | |||
| MMP1** | matrix metallopeptidase 1 | 1.05 ± 0.55 | 52.62 ± 19.16 | 2942.30 ± 1107.47 | |||
| CAV1 | caveolin 1, caveolae protein | 0.28 ± 0.02 | 4.17 ± 0.36 | 0.95 ± 0.05 | |||
| CAV2 | caveolin 2 | 0.34 ± 0.03 | 4.64 ± 1.40 | 2.31 ± 0.67 | |||
| AKT1 | v-akt murine thymoma viral oncogene homolog 1 | 1.33 | 2.22 | 3.22 | |||
| ANGPT1 | angiopoietin 1 (ANGPT1) | 0.63 | 0.07 | 6.59 | |||
| FGF1 | fibroblast growth factor 1 (acidic) | 0.66 ± 0.41 | 0.53 ± 0.68 | 2.49 ± 0.97 | |||
| FGF2 | fibroblast growth factor 2 (basic) | 0.22 ± 0.01 | 0.98 ± 0.05 | 9.38 ± 0.67 | |||
| TGFB1** | transforming growth factor, β1 | 3.59 ± 0.20 | 6.31 ± 0.28 | 10.04 ± 0.54 | |||
| VEGFA | vascular endothelial growth factor A | 0.86 ± 0.01 | 1.96 ± 0.33 | 8.88 ± 2.73 | |||
| CXCL12 | chemokine (C-X-C motif) ligand 12 | 0.20 ± 0.005 | 0.0003 ± 0.0001 | 0.03 ± 0.002 | |||
| CXCR4 | chemokine (C-X-C motif) receptor 4 | 2.87 ± 0.07 | 0.003 ± 0.0003 | 0.008 ± 0.0008 | |||
| SACTA2 | actin, α2, smooth muscle | 0.43 | 0.04 | 2.10 | |||
* Expression profiles were calculated as fold-changes compared to expression in normal tissue. Each value represents the average of more than two features from the gene chip. Data obtained from more than three features are presented with their SDs; ** These data have already been reported in our previous paper [7].