Table 2.

Env properties on high CD4 cells

Resistance pathway	Construct	Infectivity		Kinetics		N44 resistance
		Relative infectivity a,b	SEM (+/−)	T1/2 (min) c	SEM (+/−)	Relative N44 resistance a,b	SEM (+/−)
WT	WT-WT	1.00	0.28	53.13	0.61	1.04	0.14
	C1-WT	1.77	0.11	61.83	0.80	1.09	0.07
	C2-WT	1.69	0.08	61.39	2.10	1.20	0.13
	C3-WT	1.07	0.10	59.00	1.26	1.10	0.05
	C4-WT	2.21	0.11	58.91	1.17	1.23	0.03
	WT-V833Id	1.28	0.44	56.48	1.80	1.04	0.08
	WT-P714Ld	1.54	0.14	56.68	3.77	1.09	0.12
E560K	WT-E560K	0.40	0.04	58.06	1.59	1.29	0.09
	WT-C1	0.18	0.07	64.98	0.80	2.50	0.03
	C1-C1	0.51	0.15	71.13	0.64	2.37	0.03
	C4-C1	0.22	0.08	69.36	0.72	2.34	0.19
	WT-C2	0.73	0.20	66.98	2.50	2.26	0.30
	C2-C2	0.39	0.01	72.00	0.41	1.58	0.05
	C3-C2	0.83	0.30	66.69	3.12	2.14	0.07
	WT-C6	0.43	0.06	67.36	1.90	3.40	0.11
	C6-C6	0.38	0.00	65.54	2.19	3.22	0.11
Dual	C2-KKR	0.53	0.16	68.54	2.28	1.61	0.23
	C4-KKR	0.29	0.04	67.66	0.85	1.87	0.28
	WT-KKR	0.60	0.26	64.92	1.50	2.10	0.03
E648K	WT-C3	1.40	0.14	48.82	0.60	1.88	0.02
	C3-C3	1.23	0.36	54.41	1.85	1.84	0.21
	C2-C3	1.75	0.05	64.62	3.90	1.50	0.03
	WT-C4	2.35	0.65	52.92	1.35	3.59	0.16
	C4-C4	1.27	0.29	58.06	1.34	3.86	0.30
	C1-C4	0.81	0.20	65.18	1.76	2.52	0.04
	C4-C4-Te	1.23	0.39	61.33	1.91	3.39	0.42
	C5-C5	0.15	0.05	69.73	4.70	0.96	0.06
	C5-C5-Te	0.26	0.06	70.28	2.30	0.90	0nnn12

^aPerformed on U87-CD4-CCR5 cells.

^bNormalization was performed against a different WT pseudovirus lot for each independent experiment.

^cPerformed on JC53 cells.

^dMutation in C-terminal tail.

^eT indicates that the C-terminal tail mutation was removed.