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. Author manuscript; available in PMC: 2014 Oct 9.
Published in final edited form as: Clin Cancer Res. 2014 May 21;20(14):3849–3861. doi: 10.1158/1078-0432.CCR-13-1916

Figure 2.

Figure 2

Knockdown of VEGFR-2 expression by treatment with siRNA decreased the expression of EZH2 and H3K27me3 in lung adenocarcinoma cell lines stimulated by VEGF. A, and B, Western blots of VEGFR-2 (siKDR), EZH2, H3K27me3, and H3 expression in the lung adenocarcinoma cell lines HCC461 and HCC4006 upon knockdown of VEGFR-2 expression by treatment with siRNA-3 following VEGF stimulation. A, and B,, bottom panel, EZH2 mRNA expression in lung adenocarcinoma cells as determined using qRT-PCR. *P < 0.05; **P < 0.03. C, Western blot of VEGFR-2, EZH2, H3K27me3, and H3 expression in A549 cells transfected with VEGFR-2 (pKDR). C, bottom panel, EZH2 mRNA expression in lung adenocarcinoma cells as determined using qRT-PCR. *P < 0.05. Overexpression of VEGFR-2 (pKDR) increased the expression of EZH2 and H3K27 following VEGF stimulation in A549 cells.