Table 1.

Primer pairs employed in polymerase chain reaction analysis

Primer set	Primer sequence	Ta*	Target
1	5'-ACCACACACTGTACTCAGA-3'	51°C	Upstream
	5'-GTCTACGTCCTGTCCAAC-3'
2	5'-GTAGGAGGCTGAGGCGGG-3'	52°C	Exon1
	5'-GTTTCACCATGTTGGTCA-3'
3	5'-GTGAGCTACCATACTCA-3'	52°C	Exon2
	5'-CTGTGTGCACACAGTGT-3'
4	5'-GATGACTGTAATTACTTGG-3'	52°C	Exon3
	5'-ATGAGAATCCAGTA-3'
5	5'-TGTAATATGTATCTTAATCAT-3'	43°C	Exon4
	5'-TGTCAGTTATGTGGCTA-3'
6	5'-CATTATTGTGACTGCATC-3'	50°C	Exon5
	5'-AACTCTTCTGTCAGTAAC-3'
7	5'-GTAATAGATCCATTCCTCA-3'	50°C	Exon6
	5'-GCTTACAGGACATTAAG-3'
8	5'-TATATATGGACCAATAAAAC-3'	50°C	Exon7
	5'-GTCACTGGCATTCATCT-3'
9	5'ACACCAATTAATGTAATTCA-3'	52°C	Exon8
	5'-TCTATGGAAATCCTACAC-3'
10	5'-TCTGTTCTGTTTCTACAC-3'	52°C	Exon9
	5'-GATGATTAGGTCAACAC-3'
11	5'-GTGTTGACCTAATCATC-3'	48°C	Exon9
	5'-CTGCAAATAGCTTATA-3'

*Annealing temperature.