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. 2014 Oct 7;107(7):1637–1646. doi: 10.1016/j.bpj.2014.07.075

Table 1.

Nucleotide turnover times and populations of nucleotides with the slow release rate in the absence and presence of blebbistatin

Sample P2 T2 (s) P2 + BLEBB T2 + BLEBB (s)
Fast fibers 0.3 ± 0.03 250 ± 25 (20) 0.35 ± 0.05 3600 ± 500 (5)
Slow fibers 0.3 ± 0.04 150 ± 20 (15) 0.4 ± 0.05 >3600 (6)
Fast myosin 16 ± 4a 800 ± 100 (6)
Slow myosin 26b 820 ± 200 (5)

Values are expressed as the mean ± SE of 5–20 observations, as indicated by the numbers in parentheses after T2 values. T2, SRX nucleotide turnover time; P2, slow-release-rate nucleotide population.

a

ATPase rate in the absence of blebbistatin taken from Myburgh et al. (40).

b

Steady-state ATPase rate of relaxed myofibrils taken from the data of Candau et al. (41).