Table 1.
Nucleotide turnover times and populations of nucleotides with the slow release rate in the absence and presence of blebbistatin
Sample | P2 | T2 (s) | P2 + BLEBB | T2 + BLEBB (s) |
---|---|---|---|---|
Fast fibers | 0.3 ± 0.03 | 250 ± 25 (20) | 0.35 ± 0.05 | 3600 ± 500 (5) |
Slow fibers | 0.3 ± 0.04 | 150 ± 20 (15) | 0.4 ± 0.05 | >3600 (6) |
Fast myosin | — | 16 ± 4a | — | 800 ± 100 (6) |
Slow myosin | — | 26b | — | 820 ± 200 (5) |
Values are expressed as the mean ± SE of 5–20 observations, as indicated by the numbers in parentheses after T2 values. T2, SRX nucleotide turnover time; P2, slow-release-rate nucleotide population.
ATPase rate in the absence of blebbistatin taken from Myburgh et al. (40).
Steady-state ATPase rate of relaxed myofibrils taken from the data of Candau et al. (41).