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Fluorescence rotational correlation times (θ) of tC°-containing oligos at 25°C
| Oligo | pH 5.4 |
pH 8.0 |
Duplex |
|---|---|---|---|
| θ (ns) | θ (ns) | θ (ns) | |
| tC°4 | 1.9 ± 0.2 | 2.9 ± 0.2 | 8.3 ± 0.2 |
| tC°9 | 2.2 ± 0.1 | 3.1 ± 0.1 | 8.5 ± 0.1 |
| tC°10 | 2.6 ± 0.1 | 3.6 ± 0.1 | 9.3 ± 0.2 |
| tC°12 | 2.9 ± 0.2 | 3.7 ± 0.2 | 8.7 ± 0.1 |
| tC°15 | 2.7 ± 0.1 | 3.6 ± 0.4 | 8.9 ± 0.1 |
| Theoretical | 2.7 | 3.7 ± 0.1 | 8.8 |
