Table 2.

Kinetics of plastoquinone exit from the Q_p portal

Run	Exit time (with M. laminosus Chl phytyl tail, ns)	Exit time (with C. reinhardtii Chl phytyl tail, ns)	Exit time (without Chl phytyl tail, ns)
1	no exit	no exit	no exit
2	no exit	no exit	no exit
3	no exit	no exit	no exit
4	no exit	no exit	21.9
5	no exit	no exit	16.9
6	no exit	no exit	15.1
7	no exit	no exit	14.3
8	no exit	no exit	10.8
9	no exit	19.5	10.1
10	no exit	16.6	9.6
11	no exit	16.6	8.9
12	no exit	14.9	8.0
13	24.4	13.1	6.2
14	23.4	12.3	4.8
15	15.4	12.0	3.7
16	14.7	11.8	3.3
17	11.2	11.1	0.2
18	10.7	10.2	2.1
19	9.9	7.7	2.0
20	8.4	6.9	0.8
21	7.3	5.9	0.7
22	3.8	4.9	0.5
23	1.9	3.7	0.4
24	1.9	3.4	0.3
25	1.5	2.8	0.2
Avg.	10.4 ± 2.1 (13)	10.2 ± 1.2 (17)	6.4 ± 1.4 (22)

The M. laminosus cytochrome b₆f complex structure (PDB ID 2E74) was utilized for DMD simulations to determine the effect of Chl phytyl tail on plastoquinone (PQ) retention. PQ exit is defined as displacement of the PQ ring (atom C6; Fig. 1C) by 11 Å from its initial position defined proximal to the His-129 ligand of the Rieske ISP. No exit: PQ was displaced by <11 Å within the 25 ns time period of the simulation. Avg., average exit time ± standard error of mean (value in parentheses denotes trials included in calculation).