Distance distributions of proteins with and without dyes and orientational factor distribution of dyes. (From top to bottom) Radius of gyration (Rg) with and without dyes, End-to-end (E2E) distance without dyes, E2E distance with dyes, dye-to-dye (D2D) distance, and orientational factor κ2 distributions of our data and the isotropic case. (Red curves) E2E and D2D distance distributions are Gaussian chain fits; (green curves) self-avoiding chain fits. To see this figure in color, go online.