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TIRF imaging of pili dynamics. Three snapshots of the moving cell with a time difference of 0.67 s. (Bright ellipsoidal spots) Cell bodies. (Arrows) Positions of presumed pili attachment points and the direction of the pilus fiber that were manually identified in frame-by-frame analysis of the recorded movies (see the Supporting Material). Note how several pili simultaneously engage in pulling the cell. See Movie S3 in the Supporting Material.
