Skip to main content
. 2014 Oct 7;107(7):1582–1590. doi: 10.1016/j.bpj.2014.07.067

Figure 6.

Figure 6

Micrographs demonstrating the effects of fatty acid mixtures on vesicle assembly. (A) A binary mixture of 95:5 DA (C10)/MA (C14:1) assembles into visible vesicles at 25 mM (23.75 mM DA, 1.25 mM MA), above its cac of 20 mM. The individual fatty acids (right panels) do not assemble at their respective concentrations. (B) A binary mixture of 95:5 DA/OA (C18:1) produces large, plentiful vesicles at 15 mM. The OA component (5 mol %) lowers the cac of the DA from 40 mM to 500 μM. OA can still assemble into vesicles at its respective concentration (0.75 mM), but the resulting vesicles are small and in low abundance because of the low net concentration of fatty acids. All samples were prepared in POPSO buffer, labeled with the membrane stain Rhodamine 6G, and imaged at 30°C. Scale bar, 6 μm.