Fig. 1.

(A) Schematic of shotgun proteomics approach; (B) Peptide evaluation against MS/MS spectrum. Cleavage at any position can yield a left and/or right fragment, called b or y-ions respectively. The ions in the series have a different m/z, which can be located on the x-axis of the spectrum. Black peaks represent unexplained events or noise. Y-axis represents the relative abundance of respective entities.