Figure 1.

Estimation of sensitivity of HRM for detection of low levels of exon 3 SNV. A: H50Q, B: A53T, C: G51D. The undiluted heterozygous sample (50% mutation) is blue and identified by arrows, and lower levels of mutations are 20% (red), 10% (green), 5% (orange), 2.5% (purple, identified by arrowheads). Controls are shown in gray. [Color figure can be viewed in the online issue, which is available at http://wileyonlinelibrary.com.]