Figure 2.

Estimation of sensitivity of HRM for detection of low levels of SNV in other exons. A: rs144758871 (exon 4), B: rs76642636 (exon 6). The undiluted heterozygous sample (50% SNV) is blue, and SNV levels in diluted samples are 20% (red), 10% (green), 5% (orange), 2.5% (purple). Controls are shown in gray. [Color figure can be viewed in the online issue, which is available at http://wileyonlinelibrary.com.]