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Coimmunoprecipitation of Cofilin with Aur-A in Nocodazole treated M12 or NIH-3T3 cell extracts harvested at different times after release. Aur-A was immunoprecipitated using anti-Aurora A antibodies, and Cofilin was detected by immunoblotting using anti-Cofilin antibodies. Mouse IgG was used as a control. Data represents the results of three independent experiments.
