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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Aug 1;1843(11):2719–2729. doi: 10.1016/j.bbamcr.2014.07.014

Figure 8. Treatment with Aur-A Inhibitor Delayed Progression of Cells Through Prophase.

Figure 8

Immunofluorescence analysis of DMSO (A) or MLN8237 (100nM) (B) treated and nocodazole synchronized M12 cells released into mitosis for 0, 30, or 60 mins. α-tubulin (green) and Cofilin (red) were visualized by staining with anti-α-tubulin and anti-Cofilin antibodies. DNA was stained with DAPI (blue). Mitotic cells presented are enlarged images from Supplemental Figure 4. C: Quantitation of the percent of cells in interphase or mitosis. Data shows average numbers of cells counted in 20 fields each from two separate experiments. D: Quantitation of the percent of cells in each mitotic phase. Data shows average numbers of cells counted in 20 random fields each from two separate experiments. Scale bar: 5μm.