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. 2014 Jun 4;2(5):383–392. doi: 10.1002/mgg3.88

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© 2014 The Authors. Molecular Genetics & Genomic Medicine published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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(A) Overall workflow of the process followed in the SRM assay. (B) Scheme of the proteins studied and the location of the selected peptides and mutations for each protein. The three letter code design the first three amino acids of each peptide, for E1α: VDG (VDGNDVFAVYNATK) and AVA (AVAENQPFLIEAMTYR); E1β: SGD (SGDLFNCGSLTIR) and LGV (LGVSCEVIDLR); E2: LSD (LSDIGEGIR) and LSE (LSEVVGSGK); E3: ALT (ALTGGIAHLFK) and EAN (EANLAASFGK); MCAD: EEI (EEIIPVAAEYDK), TGE (TGEYPVPLIR), AFT (AFTGFIVEADTPGIQIGR), and IYQ (IYQIYEGTSQIQR). Th BD: thiamine-binding domain; L BD: lipoyl-binding domain; NAD BD: NAD-binding domain.