Table 2.
Percentages of CFTR+ hAMSCs labelled with CM-DiI in co-cultures with CFBE-F508del cells
% of CM-DiI+ CFTR+ cells | % of CFTR+ in whole CM-DiI+ population | P | |
---|---|---|---|
hAMSCs | 5.5 ± 0.8 | 5.5 ± 0.8 | _ |
CFBE-F508del | _ | 15.5 ± 0.5 | 0.0004 |
hAMSC-CFBE 1:5 | 26.4 ± 1.7 | 42.0 ± 6.3 | 0.0046 |
hAMSC-CFBE 1:10 | 18.3 ± 0.1 | 30.3 ± 7.4 | 0.0284 |
hAMSC-CFBE 1:20 | 18.2 ± 0.2 | 34.6 ± 9.3 | 0.0351 |
Percentages of CM-DiI+CFTR+ cells were obtained by plotting the FLH-1 channel, identifying CFTR-specific green signal, against FLH-2 channel, identifying red-labelled hAMSCs. Percentages of CFTR-expressing hAMSCs in whole CM-DiI+ population were obtained by dividing the double-positive hAMSCs for all CMDiI+ cells (with and without green signal). As hAMSCs are stained with CM-Dil, the same percentage is present in both columns. Data are shown as the mean ± SEM of three experiments. Significance is referred to CFTR+ cells in the whole CM-DiI+ population in all conditions as compared with hAMSCs alone.