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. 2014 Jun 3;18(8):1631–1643. doi: 10.1111/jcmm.12303

Table 2.

Percentages of CFTR+ hAMSCs labelled with CM-DiI in co-cultures with CFBE-F508del cells

% of CM-DiI+ CFTR+ cells % of CFTR+ in whole CM-DiI+ population P
hAMSCs 5.5 ± 0.8 5.5 ± 0.8 _
CFBE-F508del _ 15.5 ± 0.5 0.0004
hAMSC-CFBE 1:5 26.4 ± 1.7 42.0 ± 6.3 0.0046
hAMSC-CFBE 1:10 18.3 ± 0.1 30.3 ± 7.4 0.0284
hAMSC-CFBE 1:20 18.2 ± 0.2 34.6 ± 9.3 0.0351

Percentages of CM-DiI+CFTR+ cells were obtained by plotting the FLH-1 channel, identifying CFTR-specific green signal, against FLH-2 channel, identifying red-labelled hAMSCs. Percentages of CFTR-expressing hAMSCs in whole CM-DiI+ population were obtained by dividing the double-positive hAMSCs for all CMDiI+ cells (with and without green signal). As hAMSCs are stained with CM-Dil, the same percentage is present in both columns. Data are shown as the mean ± SEM of three experiments. Significance is referred to CFTR+ cells in the whole CM-DiI+ population in all conditions as compared with hAMSCs alone.