Table 1.

Primer sequences used in this study

Primer name	Sequence (5′–3′)
Reverse transcription-PCR
TMEM106A-F	ATGGGTAAGACGTTTTCCCAG
TMEM106A-R	TCATGGTGGGTGAGGGGT
GAPDH-F	GACCACAGTCCATGCCATCAC
GAPDH-R	TCCACCACCCTGTTGCTGTAG
Real-time PCR
TMEM106A-F	AGAGGCTGAAGCCCAAGC
TMEM106A-R	GAGGTCACCAGGCAGATGAG
GAPDH-F	TCCACTGGCGTCTTCACC
GAPDH-R	GGCAGAGATGATGACCCTTTT
Bisulphite genomic sequencing (BGS)
TMEM106A-F	GGTGGGTTTTTAGGGGTTGG
TMEM106A-R	CAACAAAAAATATCTTAACCTCC
Methylation-specific PCR (MSP)
TMEM106A-M-F	GAGTTAAAGTTTGAGGGGAGTGTTC
TMEM106A-M-R	CGTTGTAGTCGTTCGCGGGTAAGG
TMEM106A-U-F	TGAGTTAAAGTTTGAGGGGAGTGTTT
TMEM106A-U-R	TGTTGTAGTTGTTTGTGGGTAAGGTTTT