Fig. 10.

Presence of lipids in the osteocyte network observed by confocal microscopy. Confocal images of osteocytes were obtained on tibia slices fixed in formalin, cut with a high-speed rotatory tool (Dremel) and stained with Nile red as described by Fowler and Greenspan ([18]). A Zeiss Laser Scanning Microscope LSM 510 Meta confocal system attached to a Zeiss inverted microscope (Axiovert 200M; Zeiss) was used (40× oil immersion objective lens). We observed more Nile red staining in the osteocytes of the A35 group compared with those of the A25 and C groups. We observed Nile red staining in the lacuno-canalicular network in the A25 and A35 groups, with more staining in the osteocyte dendrites in the A35 group.