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. 2013 Aug 15;18(8):1680–1693. doi: 10.1111/jcmm.12113

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© 2013 The Authors. Journal of Cellular and Molecular Medicine Published by Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig. 11 — Paxillin and sclerostin mRNA expression in right tibia in control (C) rats and in both alcohol-fed (A25 and A35) rats (n = 8 in C group, n = 8 in A25 group, n = 10 in A35 group for paxillin mRNA and n = 9 in C group, n = 11 in A25 group, n = 10 in A35 group for sclerostin mRNA). Total RNA was extracted from frozen bone tissue and analysed by RT-QPCR as described in materials and methods. Paxillin mRNA expression in the A25 group was not significantly different from control group, but was higher in the A35 group compared with A25 and control groups (A35: 39.2 ± 12.1 versus A25: 16.4 ± 5.2, P < 0.05; and A35: 39.2 ± 12.1 versus C: 9.12 ± 2.23, P < 0.05). Sclerostin mRNA expression was higher in the A35 group compared with the control group: A35: 20.8 ± 9.34 versus C: 3.23 ± 2.59, P < 0.05. * indicates a significant difference (P < 0.05).