Figure 1. Breeding scheme to generate wild-type variants.

(a) Mice heterozygous for the mtDNA mutator allele (PolgA^wt/mut) were intercrossed to generate Type I (PolgA^wt/wt), Type II (PolgA^wt/mut), and Type III (PolgA^mut/mut) mice, all with inherited germline mtDNA mutations from their heterozygous (PolgA^wt/mut) mother. Type II (PolgA^wt/mut) and Type III (PolgA^mut/mut) mice also formed de novo somatic mtDNA mutations. (b) Male Type II (PolgA^wt/mut) mice were crossed with female wild-type mice to generate Type IV (PolgA^wt/wt) and Type V (PolgA^wt/mut) mice, both without inherited mtDNA mutations. Schematic adapted from Ross et al. 2013.