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. 2014 Aug 26;124(10):4240–4256. doi: 10.1172/JCI74072

Figure 4. Loss of Sorcs1 impairs proinsulin and proPC1/3 processing and increases islet insulin degradation.

Figure 4

(A) Double staining of islet cryosections for total insulin (red) and TGN38 (green) in B6 ob/ob and Sorcs1 KO ob/ob mice. (B and C) Mass spectrometry analysis of proinsulin and mature insulin content (B) and proinsulin-to-insulin ratio (C) in B6 ob/ob and Sorcs1 KO ob/ob islets. (D) Proinsulin processing in B6 ob/ob and Sorcs1 KO ob/ob islets. (E) Densitometric scanner analysis of the proinsulin and insulin bands in D. (F) Representative immunoblotting of freshly isolated B6 and Sorcs1 KO islet lysates probed for total PC1/3 and α-tubulin. (G) Quantitation of PC1/3 immunoblot as a ratio of proPC1/3 and des-PC1/3 to mature PC1/3. Data are represented as mean ± SEM.