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. 2014 Sep 9;124(10):4603–4613. doi: 10.1172/JCI74087

Figure 3. Anti-CD45RB does not induce iTregs in adoptively transferred Tconvs.

Figure 3

(A) Representative flow cytometry plots showing sort gates used to isolate FOXP3+ or FOXP3– CD4+ cells from Foxp3-RFP congenic (Thy1.1) mice (top row) and purity assessment after sort (bottom row). Numbers represent percentage of events in each gate. (B) Representative flow cytometry plots of live CD4+ cells recovered after adoptive transfer of highly purified FOXP3–CD4+Thy1.1+ cells (obtained as in A) into WT congenic mice that were then treated with control IgG or anti-CD45RB (examined in spleens on day 10). Numbers indicate average (± SD) percentage of transferred (Thy1.1+CD4+) cells recovered among total live CD4+B220– cells and FOXP3 expression within the Thy1.1+-transferred cell population. (C) Number of FOXP3–CD4+ and FOXP3+CD4+ cells from from right column in B after anti-CD45RB, relative to control IgG (n = 9 mice per group from 3 independent experiments).