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. 2014 Sep 9;124(10):4603–4613. doi: 10.1172/JCI74087

Figure 6. Anti-CD45RB specifically inhibits integrin-dependent motility of Tregs.

Figure 6

(A) Representative flow cytometry histograms of surface expression of indicated proteins on FOXP3– and FOXP3+CD4+ T cells from spleens of mice 10 days after control IgG or anti-CD45RB treatment. Gated on live CD4+B220–CD8a– lymphocytes. n = 3 mice per group. (B) Representative cell-tracking diagrams of rested FOXP3– and FOXP3+CD4+ T cells on ICAM-1–coated plates with or without the addition of anti-CD45RB in graded concentrations. Axis numbers indicate cell position from origin (μm). (C) Mean velocity and displacement values of the tracks from B. Between 21 and 84 cells were tracked in each group (from 2 independent experiments). A third independent experiment gave similar results (data not shown). (D) Mean velocity and displacement values of rested FOXP3– and FOXP3+CD4+ T cells on fibronectin-coated plates with the addition of control IgG or anti-CD45RB (1 μg/ml). Between 246 and 1,091 cells were tracked in each group (from 2 independent experiments). Horizontal bars indicate median values. *P < 0.05 vs. untreated or control IgG, **P < 0.05 vs. FOXP3–CD4+ for each condition.