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. 2014 Sep 2;124(10):4305–4319. doi: 10.1172/JCI77116

Figure 3. miR-182 deletion decreases the rate of sarcoma metastasis to the lungs.

Figure 3

(A) Schematic showing deletion of miR-182 by Ad-Cre (top panel) and PCR to show Cre-mediated excision of recombined allele from primary KP sarcomas (bottom panel). Asterisk denotes the only sample (1/25) with partial recombination of the miR-182–flox allele. (B) RT-PCR and (C) Northern blot validates deletion of miR-182 in primary sarcoma cells from KPY Mir182 mice. Asterisk denotes the only sample (1/25) with partial recombination of the miR-182–flox allele. (D) No significant change in tumor onset was observed. (E) Deleting miR-182 in primary sarcomas in mice decreases the rate of lung metastasis (Mantel-Cox log-rank test). (F) Quantification of the number of lung metastases, (G) percentage of lung area with metastasis, and (H) size of individual metastatic nodules among different genotypes. One-way Anova was used for statistical analysis. All data are mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.005.