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. 2014 Sep 2;124(10):4305–4319. doi: 10.1172/JCI77116

Figure 4. miR-182 overexpression increases the rate of sarcoma metastasis to the lungs.

Figure 4

(A) Schematic showing transgenic mice with conditional overexpression of miR-182 (top panel) and PCR to show Cre-mediated excision of the STOP cassette by Cre in primary KP sarcomas (bottom panel). (B) RT-PCR and (C) Northern blot validates significant overexpression of miR-182 in primary sarcoma cells from miR-182–overexpressor KP mice. (D) No change in tumor onset in primary sarcomas from KPY LSL-Mir182 mice. (E) Overexpression of miR-182 in primary sarcomas increases the rate of lung metastasis (Mantel-Cox log-rank test), *P < 0.05. (F) Quantification of the number of lung metastases, (G) percentage of lung area with metastasis, and (H) size of individual metastatic nodules between the 2 genotypes. Two-tailed Student’s t test is used for the statistical analysis. All data are mean ± SEM. *P < 0.05; **P < 0.01.