Figure 5. Depletion of STXBP5 in platelets affects ATP/ADP release, but not aggregation.

Aggregation (A–C and H–J) and ATP/ADP release (D–G and K–N) were monitored simultaneously in a lumi-aggregometer. Washed platelets (2.0 × 10⁵/μl) from Stxbp5 KO and WT mice were stimulated with 100 μM PAR-4 peptide (A and E), 10 nM A23187 (B and F), 0.1 μg/ml convulxin (C and G), or thrombin (Thr) at 0.025 U/ml (H and M), 0.05 U/ml (I and N), or 0.1 U/ml (J and O) for 3 minutes. (A–C, E–J, and L–N) Representative traces from 1 experiment. (D and K) Mean ± SEM ATP/ADP release from 3 independent experiments. *P < 0.01, 2-way ANOVA.