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. 2014 Oct 9;2:184. doi: 10.3389/fpubh.2014.00184

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Copyright © 2014 Neira, Mahmood, Reisen, James and Romoser.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Vacuolation of midgut epithelium. (A) Section of the posterior midgut of a specimen of the COAV strain, 3 days after ingesting a blood meal containing 5-log PFU of WEEV per 0.1 ml blood. Notice the extensive formation of vacuoles in the cytoplasm. Rusty-brown staining is indicative of a positive immunocytochemical reaction, denoting the presence of WEEV antigen in the tissue. (B) Comparable section of the posterior midgut in an uninfected control. FB, fat body; L, midgut lumen; ME, midgut epithelium; V, vacuole.