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. 2014 Oct 9;2:184. doi: 10.3389/fpubh.2014.00184

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Copyright © 2014 Neira, Mahmood, Reisen, James and Romoser.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Egg infection and pathology. (A) Longitudinal section of the abdomen of specimen of the COAV strain, 21 days after ingesting a blood meal containing 5-log PFU of WEEV per 0.1 ml blood. Rusty-brown staining is indicative of a positive immunocytochemical reaction, denoting the presence of WEEV antigen in the tissue. Notice the smooth yolk texture observed in infected egg, in contrast with the uniformly granular texture of yolk observed in the neighboring uninfected eggs. (B) Comparable section of the abdomen of a specimen of the COAV strain, showing only normal, uninfected eggs. IE, infected egg; UE, uninfected egg.