Figure 5.

Schematic diagram of constructs (A), and phenotype (I) and lignin staining (II) of wild-type and infiltrated fruits (B). (A) All constructs are in the binary vector pBI121. CaMV 35S, the 35S promoter of cauliflower mosaic virus; intron, the second intron of strawberry FaQR gene (Raab et al., 2006); NOS, terminator of the nopaline synthase gene. A control construct (pBI-intron) contained a GUS gene interrupted by an intron; an ihpRNA-cassette construct contained an intron flanked by partial coding sequences (300 bp) of target genes (FaCCR. FaCAD, or FaPOD) in sense and antisense orientations; an overexpression-cassette comprised the full-length coding sequence of target genes (FaCCR. FaCAD, or FaPOD). All constructs were used for agroinfiltration of fruits. (B) All photographs were taken 14 days after infiltration. WT (Wild type; a, c) was used as a non-infiltrated fruit. Infiltrated fruits are those injected with Agrobacterium suspensions harboring different constructs, as described in (A). (I) Phenotypes of WT (a, c) and infiltrated fruits (b, d). (II) Cross-sections of WT (a, c; n = 2) and infiltrated fruits (b, d; n = 2) before and after Wiesner staining. The arrow indicates native lignins in the fruit infiltrated with Agrobacterium suspensions harboring different constructs, as described in (A).