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. 2014 Oct 8;55(10):6265–6271. doi: 10.1167/iovs.14-14721

Figure 1.

Figure 1

Comparison of the time-dependent decrease of all-trans retinol in the conditioned media of rMC-1 incubated with bIRBP or BSA at 0, 12, and 24 hours. (A) Amount of all-trans retinol remaining in the conditioned media of cells incubated with 10 μM all-trans retinol and 2 μM BSA (♦) or bIRBP (▪). (B) Representative HPLC chromatograms of retinoid extracts from conditioned media of cells incubated with 10 μM all-trans retinol + 2uM bIRBP; retention time of all-trans retinol = 17.8 minutes (n = 9 for all data points, mean ± SE; seeding density: 250,000 cells/mL).