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. 2014 Oct 8;55(10):6265–6271. doi: 10.1167/iovs.14-14721

Figure 4.

Figure 4

High performance liquid chromatography chromatograms and UV spectra of retinols. (A) High performance liquid chromatography purified all-trans retinol with 1.1% 13-cis retinol. (B) Retinols in conditioned media with all-trans retinol + 2 μM bIRBP after 24 hours at 37°C + 5% CO2 (similar results were obtained without cells and with osteosarcoma cells; sm63- ATCC). (C) Retinol in conditioned media from cells incubated with all-trans retinol and 2 μM BSA for 24 hours. (D) Retinols in conditioned media from cells incubated with all-trans retinol and 2 μM bIRBP for 24 hours. (E) Ultraviolet spectra from online photodiode array detector for peaks 7, 8, and 9 in (D). Peak 7 is 11-cis retinol with a λmax of 318 nm. Peak 8 is identified as 13-cis retinol with a λmax of 330 nm and is consistent with spectral scans of peaks 1, 3, and 5 in (A–C). Peak 9 is identified as all-trans retinol with a λmax of 325 nm and is consistent with UV spectra of peaks 2, 4, and 6 in ([A–C]; n = 9 for all data points, mean ± SE).