Figure 3.

PDE blockade leads to enhanced insulin secretion and intracellular cAMP production. Mouse β-Min6 cells were treated with PDE inhibitor IBMX (50 μmol/l) for 2 h under low- (1 mmol/l) or high- (25 mmol/l) glucose conditions. Cells were examined for (A) insulin secretion and (B) intracellular cAMP levels (normalized to protein content; *P<0.05, **P<0.01, and ****P<0.0001). Samples were run in triplicate, and data are representative of at least three independent experiments.