Figure 4.

Resveratrol (RES) and curcumin (CUR) reduce PDE expression in β-cells. (A) Mouse β-Min6 cells and (B) human HP62 β-cells were incubated with vehicle, RES (0.1 μmol/l), or CUR (1 pmol/l) for 2 h under low- (1 mmol/l) or high- (25 mmol/l) glucose conditions. (C) Primary human islets (n=3 donors) were incubated with vehicle, RES (10 μmol/l), or CUR (100 pmol/l) for 2 h under low (5 mmol/l) or high (25 mmol/l) glucose conditions. Samples, ran in triplicate, were analyzed for relative PDE mRNA expression using quantitative RT-PCR and results are expressed as mean±s.e.m. (*P<0.05, **P<0.01, ***P<0.001, ****P<0.0001). Data are representative of at least three independent experiments.