Figure 5.

Resveratrol (RES) and curcumin (CUR) impede PDE activity in pancreatic β-cells. (A) Mouse β-Min6 cells and (B) human HP62 β-cells were cultured for 2 h under low- (1 mmol/l) or high- (25 mmol/l) glucose conditions. (C) Primary human islets (n=2 donors) were cultured for 2 h under low- (5 mmol/l) or high- (25 mmol/l) glucose conditions. Cell lysates were treated with vehicle, RES, or CUR and analyzed for relative PDE activity using a bioluminescence reaction assay. Samples were run in triplicates and the mean was plotted (*P<0.05, **P<0.01, ***P<0.001, and ****P<0.0001). Data are representative of at least three independent experiments.