Table 2.

Primers used on ABCB1 and ABCC2 fragments amplification.

GEN	EXON	Primer		MgCl2 (mM)	$T_{\mathrm{h}}^{\mathrm{o}}$	Size (bp)
		Fw	RV
ABCB1	2	5′-AGGTTAGTCTCACCTCCAGCG-3′	5′-GGCTAGCTTGCGTTTCTTAAA-3′	1.0	57	270
	11	5′-ATTCGAAGAGTGGGCACAAA-3′	5′-TCATCTCACCATCCCCTCTGT-3′	1.0	52	398
	12	5′-ATTCGAAGAGTGGGCACAAA-3′	5′-TCATCTCACCATCCCCTCTGT-3′	1.0	52	398
	14	5′-TTGGGCTGTGTATAGGATTCC-3′	5′-AAGCCTCACTGACCTTATCCA-3′	1.5	55	274
	21	5′-CAGCATTCTGAAGTCATGGAA-3′	5′-TCCAAGAACTGGCTTTGCTA-3′	1.75	56	576
	26	5′-TGTGCTGGTCCTGAAGTTGAT-3′	5′-TGGTCGAACACTTTCATCCCT-3′	1.50	62	472
ABCC2	10	5′-TTAGGCATTGACCCTATCCA-3′	5′-GCCCAAACTCCCATTAAGAA-3′	1.25	56	366
	25	5′-CGGGACTGGCTGATTCTTTA-3′	5′-ATGGGTAAATACCCAGGGGAA-3′	1.0	54	273
	28	5′-TTCTATGACACGAGTCCTGGG-3′	5′-CATCCAGGCCTTCCTTCACT-3′	1.25	56	258
	29	5′-CCCCAAGAATTATTTGTGGAA-3′	5′-GCATGTGCCCGAGTAAGTT-3′	1.5	57	238
	32	5′-GCCTAGACTTGAGATGCTGC-3′	5′-TGGGGCCTTCTGCTAGAATT-3′	1.5	55	198

The primer design was performed using the web page http://www.yeastgenome.org/cgi-bin/web-primer, $T_{\mathrm{h}}^{\mathrm{o}}$ were selected by a temperature gradient, and MgCl₂ concentration was selected initially with 2.0 mM and performed gradually until obtained a single sharp band.