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. 2014 Aug 12;42(18):e137. doi: 10.1093/nar/gku677

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Schematic overview of DNA anchoring strategies used in this study. (A) Tethering of DNA in magnetic tweezers by coupling a multiply biotinylated handle on one extremity of the DNA to a streptavidin-coated magnetic bead, and by binding a handle containing multiple digoxigenin molecules on the other extremity of the DNA to anti-digoxigenin IgG antibodies adsorbed on nitrocellulose. (B and C) Tethering of one DNA extremity via covalent coupling NH₂-enriched handles on the DNA to a surface. The other DNA extremity can then be bound to either (B) neutravidin-coated magnetic beads using labeled DNA handles containing multiple biotins or to (C) maleimide-labeled magnetic beads using a single DNA 5′ thiol-modification. In both cases (B and C) the magnetic bead coatings include PEG polymers that serve both as a passivation layer and as a covalent crosslinker.