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. 2014 Aug 12;42(18):e137. doi: 10.1093/nar/gku677

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Non-specific adhesion and spatial stability of beads. (A and B) Images of a flow cell with non-specifically adhered magnetic beads. (C) The number of adhered magnetic beads is summarized in the histogram for different combinations of bead-surface for a variety of buffer conditions. The error bars represent the mean standard deviations and the asterisks denote the significance threshold level of the applied analysis of variance (1-way ANOVA; *P < 0.05) for each buffer condition. (D) The position stability of reference beads affixed via surface-melting (black) or covalent attachment (red) is analyzed by computing their respective Allan deviations (48) as a function of time in all three dimensions. For each attachment method, the median of n = 25 reference beads is presented as continuous lines and the 25th and 75th percentiles as dashed lines.