Table 1. Sequences of the oligonucleotides used for the synthesis of the 1 kbp hairpin constructs.
Oligonucleotides | Sequences |
---|---|
U-turn | 5′CCTAAGCTCGCCGAGGCGAGCGAAAGCTCGCCTCGGCGAGCT |
Upper handle, forward | 5′GGCCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCATGCTCTTTACAACCGGTTGACTGCTTCAGGGGTCGATCCCGCTTTGTAC |
Upper handle, reversed | 5′GGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT |
Lower handle, forward | 5′GCAAGTACAAAGCGGGATCGACCCCTGAAGCAGTCAACCGGTTGTAAAGAGCATCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGA |
Lower handle, reversed | 5′GATCTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGC |