Figure 5.

R5a mobility maps reveal DNA duplex shape. (A) Plots of P_TEMPOL along BAX (black) and p21 (red) sequence. (B) Pair-wise P-values between spectra acquired at corresponding sites along the two DNAs. The CWWG core regions were shown in gray, and the central region in blue. A dashed line was drawn at P = 0.980 to aid visualization. (C) Comparisons between the P_TEMPOL map (black) and that of Roll (blue) for BAX (top panel) and p21 (bottom panel). P_TEMPOL value was aligned to the Roll value of the base-pair step 3′ of the spin-labeled phosphate. Pearson's coefficients between the two maps were 0.848 and 0.685, respectively, for BAX and p21.