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. 2014 Aug 16;42(18):e142. doi: 10.1093/nar/gku733

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Determination of response factors by usage of commercial Am and ¹³C Am from SIL-IS. (A) MS spectrum of unlabeled 2′-O-methyladenosine (Am) (left) and ¹³C-labeled Am (right). (B) Upper left: calibration measurements of commercially available, unlabeled Am. At high substance amounts, a flattening of the calibration curve due to saturation effects is highlighted in gray. Below, the signal intensity for constant amounts of ¹³C-labeled Am in the presence of increasing unlabeled Am amounts is shown. Here, the drop in signal intensity due to saturation can also be observed in the gray area. Right: By division of corresponding MS signals of unlabeled and ¹³C labeled Am, the NIF is received and a dynamic calibration curve can be plotted. The slope of the linear fit represents the relative response factor for Am = rRFN (Am).