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. 2014 Aug 16;42(18):e142. doi: 10.1093/nar/gku733

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Determination of TruB turnover completeness. Saccharomyces cerevisiae tRNA^Phe transcript was incubated with TruB, digested and SIL-IS added to determine the absolute amount of pseudouridine formed. For analysis, the MS/MS traces were used to calculate the amount of injected pseudouridine in the sample. These results were compared to the amount of injected RNA, which is received by analysis of the UV chromatogram at 254 nm. Thereby, a turnover efficiency of 99.8% was found for TruB/S.cerevisiae tRNA^Phe. Numeric details and a flow chart are given in Supplementary Table S2 and Supplementary Figure S1.